Fig. 2: In situ overall landscape of the cubic-core complex.
From: Molecular architecture of the mammalian 2-oxoglutarate dehydrogenase complex
a One slide of a representative mitochondria tomogram from all 28 tomograms. Complex with the cubic core in the mitochondria matrix was marked by the orange box. The black bar represents 100 nm. b Statistical numbers of E1o and E3 per cubic-core complex. The mean values of E1o and E3 were 4.19 and 4.36 in a complex, respectively, and the whole ratio of E1o and E3 is nearly 1:1. All of the 86 complexes were analyzed, which contain 361 E1os and 375 E3s. A boxplot shows the outliers, minimum, first quartile, medium, third quartile, and maximum of the data. c Occupancy of the peripheral subunits. The cubic-core complex is not fully occupied, and most of the complex occupancy is lower than 50%. The largest number of particles in our reconstructed complexes are those with about 30% occupancy, which is the same as the purified OGDHc.
