Fig. 5: Impact of the RNA binding activity of TRIM25 on its antiviral activity. | Nature Communications

Fig. 5: Impact of the RNA binding activity of TRIM25 on its antiviral activity.

From: The molecular dissection of TRIM25’s RNA-binding mechanism provides key insights into its antiviral activity

Fig. 5

a Red fluorescence signal in TRIM25 WT (black) and mutant lines (m3 in blue, m9 in orange and E3-dead in purple) infected with SINV-mCherry (left panel) or SINV-nsp3-mScarlet (right panel). Fluorescence was measured every 15 min in a plate reader with atmospheric control (5% CO2 and 37 °C). The fluorescence is represented as mean ± SD of six independent infections in three biological replicates. Statistical differences are based on a two-tailed homoscedastic t-test (***p < 0.001; **p < 0.01; *p < 0.05). No adjustments were made for multiple comparisons. b Localization analysis by immunofluorescence and smFISH of TRIM25 (green) and SINV RNA (red). Nuclei are labelled with DAPI. Green and red fluorescence profiles for regions of interest are displayed on the right.

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