Fig. 4: ER-mit MCSs in different brain cells and Alzheimer’s disease cell models. | Nature Communications

Fig. 4: ER-mit MCSs in different brain cells and Alzheimer’s disease cell models.

From: Simultaneous detection of membrane contact dynamics and associated Ca2+ signals by reversible chemogenetic reporters

Fig. 4

a–f Representative confocal images of different ER-mit RspA-splitFAST-expressing cells. Where indicated, cells were co-stained with MitoTracker Deep Red or mitoDsRed, used as mitochondrial marker. ER-mit MCSs are shown in mouse primary microglia (a), astrocytes (b, c), cortical neurons (d, e) and human fibroblasts (f). In (b, c, e, f) the box plots represent the percentage of mitochondrial surface co-localized with ER-mit RspA-splitFAST for the indicated cell types. In b, astrocytes from WT or AD mice were compared. In (c) astrocytes from WT mice were exposed (WT + Aβ) or not (WT) to a conditioned medium containing naturally generated Aβ peptides (see Methods). In (e) cortical neurons from WT or AD (AppNL-G-F, shortened as NLGF) mice were compared. In (f) primary human skin fibroblasts from either a healthy donor (WT) or a familial AD patient (PS2-N141I) were compared. *p < 0.05; **p < 0.01; ***p < 0.001. d Confocal microscopy 3D projection of the ER-mit RspA-splitFAST fluorescent signal in a cortical neuron from WT mice. The colour bar on the left represents the depth (in µm) along the z-axis. See also the corresponding Supplementary Movie 12. Scale bar: 10 µm (a–c, e, f); 50 µm (d).

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