Fig. 1: AA005 resists obesity and its metabolic disorders in diet-induced obese mice.
a Chemical structure of AA005. b Weight gain of male littermates fed HFD without or with AA005 (1.7 mg/kg) was recorded for 22 weeks (n = 9 for each group). c Representative photograph of vehicle and AA005-treated HFD-induced obese mice at experimental endpoint. Scale bar: 1 cm. d Fat weight was measured after 22 weeks of treatment (n = 9). e Fat weight of inguinal white adipose tissue (iWAT), abdominal subcutaneous white adipose tissue (asWAT), epididymal white adipose tissue (eWAT), retroperitoneal white adipose tissue (rWAT), mesenteric white adipose tissue (mWAT), and brown adipose tissue (BAT) in vehicle and AA005-treated obese mice (n = 9). f, Representative images of haematoxylin and eosin (H&E) stained of iWAT, eWAT, and BAT. iWAT, n = 79 (Vehicle) and 163 (AA005) adipocytes per group; eWAT, n = 90 (Vehicle) and 142 (AA005) adipocytes per group; BAT, n = 541 (Vehicle) and 392 (AA005) LDs per group. Scale bar, 50 μm. Glucose tolerance test (GTT) (g), area under the curve (AUC) of GTT (h), insulin tolerance test (ITT) (i), and AUC of ITT (j) in vehicle and AA005-treated obese mice (n = 9). k Body temperature was compared between the two groups (n = 5 per group). Three different biological replicates were tested. Mice at the end of week 21 were performed metabolic cage assays for energy expenditure (l, m), O2 consumption (n, o), and CO2 production (p, q) (n = 5). Data are presented as mean ± SEM. Unpaired, two-tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001, NS indicates not significant. Source data are provided as a Source Data file.
