Fig. 2: AA005 ameliorates obesity independent of leptin.

a Plasma leptin levels in vehicle and AA005-treated mice fed CD or HFD (n = 5). b Plasma leptin levels in wild-type (WT) and ob/ob mice treated with vehicle or AA005 (n = 5). c Body weight of WT and ob/ob male littermates treated with vehicle or AA005 (1.7 mg/kg) (n = 9 for WT mice, n = 8 for ob/ob mice for each group). d The average daily food intake of each mouse was recorded (n = 9 for WT mice, n = 8 for ob/ob mice for each group). e A representative photograph of WT and ob/ob mice treated with vehicle or AA005 at experimental endpoint. Scale bar, 1 cm. f Fat weight of iWAT, asWAT, eWAT, rWAT, mWAT, and BAT in WT and ob/ob mice treated with vehicle or AA005 (n = 9 for WT mice, n = 8 for ob/ob mice). g Fat and lean mass (n = 9 for WT mice, n = 8 for ob/ob mice for each group). h Representative images of H&E stained of iWAT, eWAT, and BAT. iWAT, n = 136 (ob/ob;vehicle) and 221 (ob/ob; AA005) adipocytes per group; eWAT, n = 93 (ob/ob;vehicle) and 165 (ob/ob; AA005) adipocytes per group; BAT, n = 546 (ob/ob;vehicle) and 384 (ob/ob; AA005) LDs per group;. Scale bar, 50 μm. Glucose tolerance test (GTT) (i), area under the curve (AUC) of GTT (j), insulin tolerance test (ITT) (k), and AUC of ITT (l) in vehicle and AA005-treated WT and ob/ob mice (n = 9 for WT mice, n = 8 for ob/ob mice for each group). Data are mean ± SEM. Unpaired, two-tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001, NS indicates not significant. Source data are provided as a Source Data file.