Fig. 2: Single-cell transcriptomic analyses of utricles treated with AAV8-CMV-, GFAP- or RLBP1-ATOH1-H2B-EGFP. | Nature Communications

Fig. 2: Single-cell transcriptomic analyses of utricles treated with AAV8-CMV-, GFAP- or RLBP1-ATOH1-H2B-EGFP.

From: Precise genetic control of ATOH1 enhances maturation of regenerated hair cells in the mature mouse utricle

Fig. 2

a Utricles from 4-10-week-old mice were treated with gentamicin (1mM) followed by CMV-ATOH1, GFAP-ATOH1 or RLBP1-ATOH1 (4 × 1011 vg/mL), and processed for single-cell RNA sequencing at day 7, 12 and 20. b UMAP plot of integrated dataset of 3 different virus treatments from all timepoints showing 6901 single cells following all quality control steps including only hair cells (HC) and supporting cells (SC). Six cell clusters including, converting SC1, converting SC2, immature HC, mature HCα and mature HCβ were identified. b’ Schematic showing supporting cell-to-hair cell conversion. c Heatmap showing differentially expressed genes among the 6 cell clusters. The top differentially expressed genes of each cluster are shown on the right. A full list of these genes is found in the source data file. The heatmap is colored by relative expression from −2 (magenta) to 2 (yellow). d–f UMAP plot colored by CMV-ATOH1 (ruby), GFAP-ATOH1 (blue) or RLBP1-ATOH1 (green) treatment. g The fraction of cells from different treatment groups and different timepoints that were assigned to each cell type. n = 7–8 utricles per treatment, per timepoint. Source data are provided as a Source Data file.

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