Fig. 3: Supporting-cell-specific promoters drive regenerated hair cell maturation ex vivo. | Nature Communications

Fig. 3: Supporting-cell-specific promoters drive regenerated hair cell maturation ex vivo.

From: Precise genetic control of ATOH1 enhances maturation of regenerated hair cells in the mature mouse utricle

Fig. 3

a Violin plot depicting ATOH1-EGFP transgene expression levels in 6 cell clusters. b UMAP plot of ATOH1-EGFP transgene expression level. c, d Schematic showing persistently and transiently upregulated ATOH1-EGFP transgene in supporting cells and regenerated hair cells after CMV-ATOH1 treatment, and GFAP-ATOH1 or RLBP1-ATOH1 treatment, respectively. e Violin plot of endogenous ATOH1 expression level in 6 different cell clusters. f UMAP plot of endogenous ATOH1 expression level. g–j Ridge plots of hair cell maturity, immaturity score, type II hair cell and type I hair cell scores of 6 different cell clusters. k Levels of expression of type I and type II hair cell genes for immature HC (yellow), mature HCα (magenta), mature HCβ (green), and developing and mature hair cells (gray). l Levels of expression of type I and type II hair cell genes for regenerated hair cells after CMV-ATOH1 (ruby), GFAP-ATOH1 (blue) or RLBP1-ATOH1 (green) treatment. Developing and mature hair cells depicted in gray. m Dot plots showing top 18 highly enriched mature hair cell genes from each cell cluster. n Dot plots showing top 10 highly enriched immature hair cell genes from each cell cluster.

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