Fig. 5: Single-cell transcriptomic analyses of IDPN-damaged utricles treated with GFAP-ATOH1 in vivo.
a 4-10-week-old mice were treated with IDPN (5 mg/g) at day 1, AAV8-GFAP-ATOH1 (1.71 × 1013 vg/mL) at day 15. Single-cell RNA sequencing was performed at 2 weeks and 1 month after injection. b UMAP plot of integrated dataset of 3 different treatment groups (control, IDPN, IDPN and GFAP-ATOH1) from both time points. There were 8399 single cells following all quality control steps including only hair cells and supporting cells. Six cell clusters including supporting cells, stage 1, stage 2, stage 3 regenerated hair cells, type II and type I hair cells were identified. c Heatmap showing differentially expressed genes among the 6 cell clusters. The top differentially expressed genes of each cluster are shown on the right. A full list of these genes is found in the source data file. The heatmap is colored by relative expression from −2 (magenta) to 2 (yellow). d–f UMAP plot colored by control (red), IDPN (green) or IDPN or GFAP-ATOH1 (blue) treatment. g The fraction of cells from different treatment groups and different time points that were assigned to each cell type. n = 6 ears per treatment per time point. Source data are provided as a Source Data file.
