Fig. 2: Modulation of PVHRUVBL2 neuron activity alters food intake.
a Schematic of stereotaxic injection of AAV9-DIO-hM3D(Gq)-mCherry or AAV9-EF1α-DIO-mCherry. b Representative images showing AAV9-DIO-hM3D(Gq)-mCherry infection and c-Fos immunoreactivity in the PVH after CNO treatment (n = 3 mice; scale bar, 100 μm). c, d Food intake of male (c) and female (d) mice post CNO injection in light cycle. AAV9-DIO-hM3D(Gq)-mCherry or AAV9-EF1α-DIO-mCherry was bilaterally injected into the PVH of chow-fed 8 week-old Ruvbl2-Cre mice and hourly food intake was recorded for 4 h post CNO injection (n = 5 mice per group). e Schematic of stereotaxic injection of AAV9-DIO-hM4D(Gi)-mCherry or AAV9-EF1α-DIO-mCherry. f Representative images showing AAV9-DIO-hM4D(Gi)-mCherry infection and c-Fos immunoreactivity in the PVH after CNO treatment (n = 3 mice; scale bar, 100 μm). g, h Food intake of male (g) and female (h) mice post CNO injection in light cycle. Cre-dependent AAV9-DIO-hM4D(Gi)-mCherry or AAV9-DIO-mCherry was bilaterally injected into the PVH of chow-fed 8 week-old Ruvbl2-Cre mice and hourly food intake was recorded within 4 h post CNO injection (n = 5 mice per group). i Schematic of stereotaxic injection of AAV9-DIO-hChR2-mCherry or AAV9-DIO-mCherry. j Procedure of optogenetic stimulation. PVHRUVBL2 neurons were stimulated by a 465 nm blue laser with 10 ms pulse duration for 10 min. Food intake in 10 min of laser stimulation, and 10 min before or after laser stimulation was monitored, respectively. Stim, during laser stimulation; Pre, before laser stimulation; Post, after laser stimulation. k Representative image showing AAV9-DIO-hChR2-mCherry infection in the PVH (n = 5 mice; scale bar, 100 μm). l Food intake of fasted mice before and after optogenetic activation of PVHRUVBL2 neurons. ChR2 group (n = 5 mice); Control group (n = 3 mice). The experiment was repeated once using the same mice and all the data were included for quantitation. Two-tailed unpaired Student’s t-test for (c–l). ns, no significance. All data are presented as means ± SEM. Source data are provided as a Source Data file.
