Fig. 5: RUVBL2 KO in the PVH impairs the excitatory synaptic transmission.

a Schematic of AAV injection into the PVH and electrophysiology recordings in the ARC. b Representative traces of sEPSCs recorded in the ARC in the presence of PTX. c, d Amplitude (c) and frequency (d) of sEPSCs recorded in ARC neurons. AAV2-GFP (n = 45 neurons/4 mice), AAV2-Cre-GFP (n = 42 neurons/4 mice). e Representative traces of eEPSCs recorded in ARC neurons in response to paired-pulse stimulations (50 ms interval) in the presence of PTX. f, g Amplitude of the first EPSCs (f) and PPR (g) recorded in ARC neurons. AAV2-GFP (n = 45 neurons/4 mice), AAV2-Cre-GFP (n = 42 neurons/4 mice). h Representative traces of sIPSCs recorded in ARC neurons in the presence of CNQX and D-APV. i, j Amplitude (i) and frequency (j) of sIPSCs recorded in ARC neurons. AAV2-GFP (n = 32 neurons/3 mice), AAV2-Cre-GFP (n = 38 neurons/3 mice). k Schematic of AAV injection into the PVH and electrophysiological recordings in DMH neurons. l Representative traces of sEPSCs recorded in DMH neurons in the presence of PTX. m, n Amplitude (m) and frequency (n) of sEPSCs recorded in DMH neurons. AAV2-GFP (n = 31 neurons/3 mice), AAV2-Cre-GFP (n = 32 neurons/3 mice). o Representative traces of eEPSCs recorded in DMH neurons in response to paired-pulse stimulations (50 ms interval) in the presence of PTX. p, q Amplitude of the first eEPSCs (p) and PPR (q) recorded in DMH neurons. AAV2-GFP (n = 30 neurons/3 mice), AAV2-Cre-GFP (n = 30 neurons/3 mice). r Representative traces of sIPSC recorded in DMH neurons in the presence of CNQX and D-APV. s, t Amplitude (s) and frequency (t) of sIPSCs recorded in DMH neurons. AAV2-GFP (n = 21 neurons/2 mice), AAV2-Cre-GFP (n = 20 neurons/2 mice). Two-tailed unpaired Student’s t-test for (c–t). ns, no significance. All data are presented as means ± SEM. Source data are provided as a Source Data file.