Fig. 6: RUVBL2 KO impairs the excitatory synaptic transmission to ARCPOMC or ARCNPY neurons. | Nature Communications

Fig. 6: RUVBL2 KO impairs the excitatory synaptic transmission to ARCPOMC or ARCNPY neurons.

From: Paraventricular hypothalamic RUVBL2 neurons suppress appetite by enhancing excitatory synaptic transmission in distinct neurocircuits

Fig. 6

a Schematic of AAV injection into the PVH and electrophysiology recordings in ARCPOMC neurons. b Representative traces of sEPSCs recorded in ARCPOMC neurons in the presence of PTX. c, d Amplitude (c) and frequency (d) of sEPSCs recorded in ARCPOMC neurons. AAV2-mCherry (n = 30 neurons/3 mice), AAV2-Cre-mCherry (n = 25 neurons/3 mice). e Representative traces of eEPSCs recorded in ARCPOMC neurons in response to paired-pulse stimulations (50 ms interval) in the presence of PTX. f, g Amplitude of the first EPSCs (f) and PPR (g) recorded in ARCPOMC neurons. AAV2-mCherry (n = 38 neurons/4 mice), AAV2-Cre-mCherry (n = 23 neurons/3 mice). h Schematic of AAV injection into the PVH and electrophysiology recordings in ARCNPY neurons. i Representative traces of sEPSCs recorded in ARCNPY neurons in the presence of PTX. j, k Amplitude (j) and frequency (k) of sEPSCs recorded in ARCNPY neurons. AAV2-mCherry (n = 33 neurons/3 mice), AAV2-Cre-mCherry (n = 29 neurons/3 mice). l Representative traces of eEPSCs recorded in ARCNPY neurons in response to paired-pulse stimulations (50 ms interval) in the presence of PTX. m, n Amplitude of the first EPSCs (m) and PPR (n) recorded in ARCNPY neurons. n = 29 neurons/3 mice per group. o Schematic of simultaneously activating ARC-projecting PVHRUVBL2 neurons and ARCNPY neurons. p Food intake of mice within 1 h after different treatments as indicated (as in o). n = 8 mice per group. q Food intake of mice within 6 h after different treatments as indicated. SHU9119, 0.5 mg/kg. Stim, optical stimulation for 6 h when the laser light (465 nm) was switched on every other 30 min. For SHU9119+Stim, mice were first intraperitoneally injected with SHU9119, followed by optical stimulation for 6 h when the laser light (465 nm) was switched on every other 30 min. n = 6 mice per group. Two-tailed unpaired Student’s t-test for (c–n). ns, no significance. One-way ANOVA with Geisser-Greenhouse correction followed by Fisher’s LSD test for (p) and (q). All data are presented as means ± SEM. Source data are provided as a Source Data file.

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