Fig. 4: The m1G9-state of the A3243G and T3271C variants is impeded for aminoacylation.
a An illustration of human mt-Leu(UAA) folding from a meta-stable intermediate-state to the chargeable-state for aminoacylation. The rate constants 0.66 min−1 and 10–20 s−1 were from published reports29,64,65,66,67,68, respectively. b The fraction of the charged tRNA as a function of time was monitored for the m1G9-state of the WT tRNA, the G9-state of the WT tRNA, the G9-state of the A3243G variant, and the m1G9-state of the A3243G variant. For each tRNA, the rate constant (k1) and the amplitude of the first phase (amp1) are shown as the average ± SD (n = 3). c The fraction of the charged tRNA as a function of Mg(OAc)2 concentration for the 4 tRNAs described in (b). Each value was the average ± SD (n = 3). d The fraction of the charged tRNA as a function of time was monitored for the m1G9-state of the WT tRNA, the G9-state of the WT tRNA, the G9-state of the T3271C variant, and the m1G9-state of the T3271C variant. For each tRNA, the rate constant (k1) and the amplitude of the first phase (amp1) are shown as the average ± SD (n = 3). e The fraction of the charged tRNA at 8 min as a function of Mg(OAc)2 concentration for the 4 tRNAs described in (d). Each value was the average ± SD (n = 3). Source data are provided as a Source Data file.
