Fig. 6: SUMO-SIM interaction between PIDD1 and RAIDD drives PIDD1 nucleolar incorporation. | Nature Communications

Fig. 6: SUMO-SIM interaction between PIDD1 and RAIDD drives PIDD1 nucleolar incorporation.

From: Stepwise phosphorylation and SUMOylation of PIDD1 drive PIDDosome assembly in response to DNA repair failure

Fig. 6

a–i HeLa cells of indicated genotypes and transfected with indicated FLAG-PIDD1 or VSV-RAIDD variants (c–i) were treated with MMC and Go6976 (1 μM each), fixed at indicated time points (hours; (a, b)) and stained with indicated antibodies and DAPI to mark nuclei (contours marked by dashed white line in (a)). At least 40 cells per sample were scored in each of n = 2 independent experiments. Representative images (a, c) were analyzed for percentage of cells with nucleolar PIDD1pT788 signals (d, f), Pearson’s coefficient (b, e, g) and distribution of PIDD1pT788 signal intensity (h, i). Source data are provided as a Source data file.

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