Fig. 7: SUMO-SIM interaction between PIDD1 and RAIDD is essential for endogenous PIDDosome formation. | Nature Communications

Fig. 7: SUMO-SIM interaction between PIDD1 and RAIDD is essential for endogenous PIDDosome formation.

From: Stepwise phosphorylation and SUMOylation of PIDD1 drive PIDDosome assembly in response to DNA repair failure

Fig. 7

a–e Parent and RAIDD—/— HeLa cells transfected with indicated VSV-RAIDD variants were treated with or without MMC and Go6976 (1 μM each), fixed at 24 h and stained with indicated antibodies and DAPI. At least 20 cells per sample were scored in each of n = 3 independent experiments. Representative images (a) were analyzed for triple colocalization count (b), triple colocalization area (c), percent area of triple signal overlaps (d) and overall cleaved C2 signal intensity (e). Data expressed as means +/− SD. *p < 0.05; **p < 0.005; ***p < 0.001; ns, non-significant, two-tailed Student’s t-test. f Three-step model for PIDDosome formation (see text). Source data are provided as a Source data file.

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