Fig. 5: CryoEM structures of the ABC transporter core of PCAT1, PCAT1_CC.

Unlike the full length PCAT1, binding of Mg²⁺ (panel A, panel B with sub bound, panel C with ATP and Vi bound) does not stabilize the IF conformation in the cryoEM grids. ATP in the absence of Mg²⁺ stabilizes the OF conformation (panel D).