Fig. 5: Elevated TRPM2 expression involved in FPP-induced inflammatory response in BD-PMN.

A Differential expression analysis of TRP channels in BD and HC PMN transcriptome sequencing data (GSE205867). Channels with significantly elevated (p < 0.05 and log2FC > 0.5) and decreased expression (p < 0.05 and log2FC > 0.5) are marked in red and blue, respectively. B The quantification of TRPM2 expression on PMN from BD and HC verified by qRT-PCR (N = 9) C Representative and summary of Western blot (WB) analysis of the TRPM2 expression in PMN from BD and HC (N = 6). D The mRNA expression levels of TRPM2 in active BD patients and those in remission (N = 14). E, F The expression of TRPM2 was silenced by TRPM2-specific siRNA, and the levels of proinflammatory cytokines (N = 6, E and NETs-dsDNA (N = 5, F) in FPP-stimulated BD-PMN were analyzed respectively. A scramble siRNA was used as a negative control. G, H Representative immunofluorescence staining diagrams (G) and quantification (N = 15, H) of 60 μg/mL FPP-stimulated PMN after TRPM2-specific siRNA or si-scramble treatment, scale bars =10 µm. I The VECs were stimulated with supernatants from 60 μg/mL FPP-pretreated PMN, after TRPM2-specific or scramble siRNA transduction. The transcriptional expression levels of the above-mentioned proinflammatory cytokines and adhesion molecules in VECs were detected by qRT-PCR (N = 6). N for all experiments were biological replicates. Data are presented as mean ± SD; error bars indicate the SD. A two-sided p-value < 0.05 was considered statistically significant, with *p < 0.05, **p < 0.01, and ***p < 0.001, indicating significant differences. Source data are provided as a Source Data file. Independent t-tests were used in B–D and F, H. Two-way ANOVA tests were used in E and I, with the p-value adjusted for multiple comparisons by FDR using the two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli.