Fig. 2: Forebrain conditional transgenic mice (H11-LSL-NEK4^±; CaMKIIα-Cre) exhibited abnormal circadian fluctuations in emotional behaviors.

A Time scheme for mouse behavioral tests in NEK4 cTG mice. B Results of open field tests during the day and night of the NEK4 cTG mice compared to the control mice, including the total distance travelled, the distance spent in the center (day: P = 0.0393, df = 14, t = 2.272; night: P = 0.6931, df = 14, t = 0.403) and time spent in the center, and the time spent in the corner, were recorded and analyzed. C Results of the elevated plus maze during the day and night of the NEK4 cTG mice compared to the control mice, and their total distance traveled (day: P = 0.0008, df = 14, t = 4.280; night: P = 0.5681, df = 14, t = 0.585), distance spent in the open arms (day: P = 0.0019, df = 14, t = 3.801; night: P = 0.2647, df = 14, t = 1.162), time spent in the open arms (day: P = 0.0018, df = 14, t = 3.833; night: P = 0.0131, df = 14, t = 2.839) and time spent in the closed arms (day: P = 0.0006, df = 14, t = 4.363; night: P = 0.0238, df = 14, t = 2.535) were calculated. D The NEK4 cTG mice and the control mice underwent the forced swimming tests and monitored for 8 min, and the immobility time in the remaining 6 min was calculated for each mouse. N = 8 mice/group. All data were shown as mean ± SD, the two-sided student’s t test was used to perform the significance test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P < 0.0001. The shaded part represents the behavioral experiment data carried out in the dark environment at night (21:00-24:00). Source data are provided as a Source data file.