Fig. 7: Lithium treatment ameliorated the circadian fluctuations in synaptic structures in NEK4 OE mice.
From: NEK4 modulates circadian fluctuations of emotional behaviors and synaptogenesis in male mice
A Densities of various types of dendritic spines in apical dendrites neurons of dorsal hippocampus CA1 from NEK4 OE mice and the control mice treated with either vehicle or lithium. Dendritic spines of the control group compared with NEK4 OE group (day: thin: P = 0.0306, df = 138, q = 3.941; night: thin: P = 0.0417, df = 147, q = 3.774. Day: mushroom: P = 0.0085, df = 138, q = 4.559; night: mushroom: P < 0.0001, df = 147, q = 8.667; 2-way ANOVA with Tukey’s multiple comparisons test). N = 12 neurons in control-day; 13 neurons in NEK4 OE-day; 12 neurons in control-Li-day; 13 neurons in NEK4 OE-Li-day; 13 neurons in control-night; 13 neurons in NEK4 OE-night; 13 neurons in control-Li-night; 14 neurons in NEK4 OE-Li- night. B Effect of lithium treatment on synaptic formation in mice. synaptogenesis during the day compared with the night in control group (P = 0.0068, df = 56, t = 2.809; two-sided student’s t test); synaptogenesis of the control group compared with NEK4 OE group without lithium treatment (day: P = 0.0035, df = 55, t = 3.056; night: P = 0.0027, df = 56, t = 3.144; two-sided student’s t test). N = 28 images in control-day; 29 images in NEK4 OE-day; 31 images in control-Li-day; 28 images in NEK4 OE-Li-day; 30 images in control-night; 28 images in NEK4 OE-night; 28 images in control-Li-night; 29 images in NEK4 OE-Li-night. C Effect of lithium treatment on PSD thickness in mice. PSD thickness during the day compared with the night in control group (P = 0.0126, df = 43, t = 2.604; two-sided student’s t test); PSD thickness of the control group compared with NEK4 OE group without lithium treatment (day: P = 0.0173, df = 38, t = 2.489; night: P < 0.0001, df = 45, t = 4.940; two-sided student’s t test). N = 20 images in control-day; 20 images in NEK4 OE-day; 21 images in control-Li-day; 22 images in NEK4 OE-Li-day; 25 images in control-night; 22 images in NEK4 OE-night; 21 images in control-Li-night; 21 images in NEK4 OE-Li-night. All data were shown as mean ± SD, P < 0.05 after corrections was defined as significant. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P < 0.0001. Shadows represent data at night. Source data are provided as a Source data file.
