Fig. 5: Interaction of TrkBtm with various drugs.

a CSPs induced in TrkBtm by FLX (empty bars) and CPR (black bars). CSPs for the separate signals of the dimer are plotted at the bottom in a mirrored scale. Peak width (PW) of the TrkBtm signals is shown as a black dotted line. The maximal CSPs are shown by * symbols (See the Statistics subsection in the Methods section). b CSPs of V437 and Y434 plotted as a function of FLX (empty circles) and CPR (black circles) concentrations for the monomer and dimer forms of TrkBtm. The dashed lines indicate the results of a third degree polynomial approximation. TrkBtm dimer (left) and monomer (right) painted according to the amide CSP values induced by an 8:1 excess of FLX (c) or CPR (d). Residues with CSP higher than 0.05 ppm are shown in black. e The signal of the F445 backbone amide in the intact sample (left) and with 4:1 excess of either FLX (middle) or CPR (right). Slices along the ¹H direction are shown on top with the corresponding signal to noise ratios (SNR) given. f Fraction of drugs residing in bicelles calculated from lateral diffusion at a drug:protein ratio of 8:1. Error bars indicate the error of the measurement. g Relative changes in the signal intensities of monomer (orange), dimer (blue), and overlapped (green) signals of TrkBtm upon drug addition. Error bars indicate the SD among the traced n = 5 NMR signals for each form. Star labels indicate statistically significant differences in the monomer and dimer populations according to the Mann-Whitney test (*p ≤ 0.05, **p ≤ 0.01), the p-values are provided in Supplementary Table 1. The relative intensities of the monomer and dimer are plotted until the signals overlap. Statistical analyses are listed in Supplementary Table 1. Source data are provided as a Source Data file.