Fig. 4: AtHIR2 and AtHIR4 play essential roles in resistance to necrotrophic fungi.

a Virulence assay of S. sclerotiorum WT on Col-0, hir4 mutant and oxSsPEIE1 lines at 30 hpi. b Lesion areas by the cross-over method in (a), n  =  8 biologically independent samples. c Relative biomass in (a) analyzed by qPCR (n = 3 biologically repetitions). d Virulence assay of B. cinerea WT on Col-0, hir4 and oxSsPEIE1 lines at 36 hpi. e Lesion area by the cross-over method in (d), n  =  12 biologically independent samples. f Relative biomass in (d) analyzed by qPCR (n = 3 biologically repetitions). g Virulence assay of S. sclerotiorum WT and ΔSsPEIE1 mutants on Col-0 and hir4 mutant at 36 hpi. h Lesion area by the cross over method in (g), n  =  10 biologically independent samples. i Relative biomass in (g) analyzed by qPCR (n = 3 biologically repetitions). j Expression of four AtHIRs in A. thaliana at different time points after S. sclerotiorum inoculation (hyphal fragment spray) using qPCR (n  =  3 biologically repetitions). AtUBQ5 was used as the reference. k Virulence assay of S. sclerotiorum WT on Col-0, hir2, hir4 and hir24 mutant at 30 hpi. l Lesion areas by the cross-over method (n  ≥  13 biologically repetitions). m Relative biomass in k analyzed by qPCR (n = 3 biologically repetitions). n Virulence assay of S. sclerotiorum WT and ΔSsPEIE1 mutants on Col-0 and hir24 mutant at 36 hpi. o Lesion area by the cross-over method (n  = 12 biologically repetitions). p Relative biomass in (n) analyzed by qPCR (n = 3 biologically repetitions). q MAPK activation induced by chitin in Col-0 and hir2, hir4 and hir24 mutants. r ROS burst induced by chitin in Col-0 and hir2, hir4 and hir24 mutant, values represent means ± SEM (n = 12 biological replicates). Data represent means ± SD except for (r). Different letters on the same graph indicate statistical significance at p < 0.01 in one-way ANOVA. Source data are provided as a Source Data file.