Fig. 4: F. sacchari PD1T GHs are active on diverse polysaccharides with high temperature optima. | Nature Communications

Fig. 4: F. sacchari PD1T GHs are active on diverse polysaccharides with high temperature optima.

From: Genome-guided isolation of the hyperthermophilic aerobe Fervidibacter sacchari reveals conserved polysaccharide metabolism in the Armatimonadota

Fig. 4

a Activity of F. sacchari GHs expressed in E. coli and screened against 15 polysaccharides. Red color represents activity compared with negative controls containing pET21b without a GH gene (n = 1). Asterisks show activities confirmed after repeating in triplicate (n = 3 experimental replicates; unpaired student’s t test p < 0.05). No adjustments were made for multiple statistical tests because significance was confirmed in triplicate separately versus the empty-vector control. b–e Temperature optima for selected GHs, tested after one hour of incubation compared to negative controls (gray circles). Boxes represent 25th and 75th percentiles and central mark is the mean. Shading shows optimal enzyme activity (n = 3 experimental replicates; ANOVA with post-hoc Tukey’s HSD p < 0.05). Source data and p-values are provided as a Source Data file.

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