Fig. 6: ZIKV-NS2AK56R infection causes weakened pathogenicity and lethality in mice.
From: AMFR-mediated Flavivirus NS2A ubiquitination subverts ER-phagy to augment viral pathogenicity
a Daily weights of mice treated with PBS (n = 6), ZIKV-NS2AWT (n = 7), or ZIKV-NS2AK56R (n = 7) (i.p., 104 PFU/ mouse); Error bars present as the mean ± SEM; Source data are provided as a Source Data file. b Survival of mice from (a); Log-rank (Mantel-Cox) test. c–g qRT-PCR analysis of ZIKV-E RNA in different tissues of mice infected with ZIKV-NS2AWT or ZIKV-NS2AK56R (n = 6); Error bars of (c–g) present as the mean ± SEM; Statistical analysis of (c–g) was performed using two-tailed unpaired t-test analysis; Source data are provided as a Source Data file. h Immunofluorescence analysis of brains of mice infected with ZIKV-NS2AWT or ZIKV-NS2AK56R. Scale bars, 1000 μm. ZIKV-E (green), DAPI (blue). i Hematoxylin and eosin staining analysis of brains of mice infected with PBS, ZIKV-NS2AWT, or ZIKV-NS2AK56R. The black arrows represent the inflammation damage in the brain tissues. Scale bars, 100 μm. j Western blotting analysis of mFAM134B expression in mouse tissues infected with ZIKV-NS2AWT or ZIKV-NS2AK56R.
