Fig. 1: Schematic summary of model for long-term perfusion of human split livers using normothermic machine perfusion.

After back table dissection, whole livers are resuscitated using normothermic machine perfusion for 12–24 hours. The liver is then surgically split without interruption to perfusion, and the two partial grafts are transferred to separate perfusion machines. The partial grafts are then perfused in the long-term, which allows for a number of experimental models, including testing therapeutics and the study of liver injury and liver regeneration. Surgery icon created by Yoyon Pujiono, https://thenounproject.com/icon/surgery-3681231/. Licensed under: https://creativecommons.org/licenses/by/3.0/”.