Fig. 7: PU.1 Knockdown in ALL-54S in vivo. | Nature Communications

Fig. 7: PU.1 Knockdown in ALL-54S in vivo.

From: PU.1 eviction at lymphocyte-specific chromatin domains mediates glucocorticoid response in acute lymphoblastic leukemia

Fig. 7

Engraftment (A) and Event-free Survival (EFS) curves (B) of ALL-54S cells with BIM KO, PU.1 KO or Non-Targeting Control (NTC) guide RNA in mouse peripheral blood (PB). Gene KO was induced by doxycycline (Dox) feeding. Leukemia event is defined as 25% human cells in mouse PB. Gene KO was induced by doxycycline (Dox) feeding. Leukemia event is defined as 25% human cells in mouse PB. Statistics were performed using log-rank (Mantel-Cox) test, n = 6/group except for the BIM KO DEX-treated group has n = 5 due to mouse loss from non-leukemia related disease. Insertion and deletion profiles of relapsed BIM KO (C) and PU.1 KO (D) ALL-54S clones following saline (Ctrl)/DEX treatment in vivo. Genomic DNA of PDX cells from two independent mice were sequenced and representative profiles from each group were shown. The TIDE webtool (http://tide.nki.nl) was used for sequencing data analysis. See also Supplementary Fig. 7. Source data are provided as a Source Data file.

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