Fig. 2: Characterization of synXIII.
From: The de novo design and synthesis of yeast chromosome XIII facilitates investigations on aging
a Scanning Electron Microscopy (SEM) images of BY4741 and synXIII strains. Scale bars: 5.00 μm. Source data are provided as a Source data file. b Growth curve analysis of BY4741 and synXIII. Each strain was analyzed with three biological replicates (n = 3), and each biological replicate consisted of three technical replicates. The error bands represent a total of nine replicates at each time point. Data are presented as mean values ±SD. Source data are provided as a Source data file c Doubling times of corresponding strains in YPD medium, each strain was conducted with three replicates (n = 3). Each replicate represents the mean doubling time of three technical replicates. Data are presented as mean values ±SD from three independent replicates. Source data are provided as a Source data file. d Cellular viability responses of synXIII upon exposure to normal and stress conditions. 10-fold serial dilutions of overnight cultures of synXIII and wild-type (BY4741 and BY4742) strains were used for plating. Conditions include: YPD at 30 °C; SC at 30 °C; YPD + Benomyl; YPD + H2O2 (1 mM, 2 h pretreatment); YPD + methyl methane sulfone (MMS, 0.01% v/v); YPD + Camptothecin (0.1 μg/mL); YPD + Rapamycin (0.2 μg/mL); YPD + Cycloheximide (10 μg/mL, 2 h pretreatment); YPD + Sorbitol (1 M); YPD, yeast extract peptone dextrose; YPEG, yeast extract peptone glycerol ethanol; SC, synthetic complete. e Survive curves of synXIII and BY4741. X-axis presents the number of daughter cells generated from the mother cell. Each strain was measured with three replicates (n = 3) and 40 cells were monitored for each replicate. Source data are provided as a Source data file. f Identified dysregulated genes of synXIII strain at the transcriptome level compared with BY4741. The P value was calculated by DESeq2 (v1.30.1). The differentially expressed genes were assessed at genome-wide significance P < 7.56 × 10−6 for 5% family-wise error rate based on 6607 genes with at least one mapped read and also corresponding to log2 (foldchange) ≥1 or ≤−1. Source data are provided as a Source data file. g Identified dysregulated genetic features of synXIII strain at the proteome level compared with BY474. The P value was calculated by IQuant (v2.0.1) in two-sided T-test. Source data are provided as a Source data file. f, g the up and down-regulated genes are labeled in red and green respectively.
