Fig. 2: ICI-induced SJS/TEN–related changes in macrophage/monocyte/myeloid cell functions and gene expression profiles. | Nature Communications

Fig. 2: ICI-induced SJS/TEN–related changes in macrophage/monocyte/myeloid cell functions and gene expression profiles.

From: Immune checkpoint inhibitor-induced severe epidermal necrolysis mediated by macrophage-derived CXCL10 and abated by TNF blockade

Fig. 2

a Sub-clustering of macrophage/monocyte/myeloid cells selected from patients with ICI-induced SJS/TEN, ICI-induced mild cADR, and control participants. A total of 31,046 cells were defined as macrophage/monocyte/myeloid cells by SingleR annotation and well-studied marker genes. b Violin plots display the marker genes of macrophage/monocyte/myeloid cell clusters. For each cluster, the selected top 10 genes of the highest expression levels with cell type–specificity score, and a highly expressed well-defined subset marker are shown. The y-axis represents normalized values for different gene expression levels (detailed gene expression profiles for different clusters are presented in Supplementary Fig. 2a). c Frequencies of macrophage/monocyte/myeloid cells in each cluster for each enrolled patient with ICI-cADR and control participants. ISB: ICI-induced SJS/TEN lesional BC; ISP: ICI-induced SJS/TEN PBMC; IMP: ICI-induced mild cADR PBMC; ITP: ICI-tolerant PBMC; HD: healthy donors PBMC. d Distributions of macrophage/monocyte/myeloid cells across clusters among patients with ICI-cADR patients and control participants. Numbers of cells defined as macrophage/monocyte/myeloid cells by group were ISB: 5579 cells; ISP: 7558 cells; IMP: 984 cells; ITP: 3247 cells; HD: 13,678. e Ranking of the significant and relevant differentially expressed genes (DEG) in macrophage/monocyte/myeloid cells comparing between ICI-SJS/TEN lesional BC and HD PBMC. f Ranking of the significant and relevant DEG in macrophage/monocyte/myeloid cells comparing between ICI-SJS/TEN lesional BC and ICI-tolerant (tolerant) PBMC. The significance of DEG was defined a using a two-sided non-parametric Wilcoxon rank-sum test and Bonferroni correction. g Functional enrichment analysis of significant and relevant hallmark gene sets identified as differentially expressed genes in macrophage/monocyte/myeloid cells comparing between ICI-SJS/TEN lesional BC and HD PMBC. NES, normalized enrichment score. h Functional enrichment analysis of significant and relevant hallmark gene sets identified as differentially expressed in macrophage/monocyte/myeloid cells comparing between ICI-SJS/TEN lesional BC and ICI-tolerant (tolerant) PMBC. All significant the significant and relevant differentially expressed genes and hallmark gene sets are shown in the source data.

Back to article page