Fig. 2: Single-cell RNA sequencing of hPSC-derived SANLPCs reveals transcriptomic similarities to fetal SAN pacemaker cells. | Nature Communications

Fig. 2: Single-cell RNA sequencing of hPSC-derived SANLPCs reveals transcriptomic similarities to fetal SAN pacemaker cells.

From: Single-cell transcriptome analysis reveals CD34 as a marker of human sinoatrial node pacemaker cardiomyocytes

Fig. 2

a Schematic overview of SANLPC differentiation protocol and sample processing for scRNA-seq on the 10x Genomics Chromium platform. b UMAP of day 25 HES2-derived SANLPCs showing 11 cell clusters (left). Subclustering of the TNNT2+ cardiomyocytes showing 6 sub-clusters (right). c UMAPs of the subclustered cardiomyocytes showing the expression of the indicated genes. d UMAPs showing signature score distribution for the DEGs of the indicated fetal SAN cell types. e Heatmap of the top 5 DEGs within the indicated cardiomyocyte subclusters. f GO analysis (biological processes) of all genes enriched in the Core SAN cluster. g UMAPs showing the top 10 DEGs of the core SAN cluster. h UMAPs showing Harmony integration of the fetal SAN snRNA-seq data and the hPSC scRNA-seq data labeled by source dataset (left), cluster number (center), and assigned cell types (right). i Stacked bar graph showing the frequency of each cell type from both fetal and hPSC datasets in the indicated cell clusters. j UMAPs of integrated fetal SAN and hPSC datasets showing the expression of the indicated genes. k Spearman correlation between selected clusters from the fetal SAN and hPSC datasets. p < 0.05 for all correlations (asymptotic t approximation). Schematics in (a) were generated using Biorender (https://biorender.com).

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