Fig. 7: CITE-seq of hPSC-derived SANLPCs shows that CD34⁺NKX2–5⁺ and CD34⁻NKX2–5⁻ cardiomyocytes are transcriptionally similar to SAN pacemaker cells.

a UMAP of the day 25 HES2-derived SANLPC CITE-seq dataset showing 11 cell clusters (left). Subclustering of the TNNT2⁺ cardiomyocytes showing 7 sub-clusters (right). b UMAPs of the subclustered cardiomyocytes showing the expression of the indicated genes. c UMAPs showing signature score distribution for the DEGs of the indicated fetal SAN cell types. d UMAPs showing the expression of CD34 on the transcript (RNA) or protein level (antibody-derived tag (ADT)) (left) and co-expression with NKX2–5 transcript (right). e Stacked bar graph quantifying the number of cells expressing CD34 by transcript (RNA) or protein (ADT) grouped based on NKX2–5 expression by transcript. f UMAPs showing the distribution of the selected cell populations indicated above. g Box plots showing the expression of the indicated genes on the transcript level and CD34 on the protein level (ADT) in the selected cell populations indicated below (CD34⁺NKX2–5⁺ n = 78; CD34⁺NKX2–5⁻ n = 1465; CD34⁻NKX2–5⁻ n = 2294; Atrial CD34⁻NKX2–5⁺ n = _97 single cells). h Spearman correlation between the selected cell populations indicated on the axes. p < 0.05 for all correlations (asymptotic t approximation). Blue labels indicate the data that is showing protein level (ADT)-based CD34 expression. Box plot elements: center line, median; box limits, upper and lower quartiles; whiskers, 1.5x interquartile range. Source data are provided as a Source Data file.