Fig. 4: Direct RNA sequencing results of E. coli samples exposed to streptomycin and kasugamycin. | Nature Communications

Fig. 4: Direct RNA sequencing results of E. coli samples exposed to streptomycin and kasugamycin.

From: Native RNA nanopore sequencing reveals antibiotic-induced loss of rRNA modifications in the A- and P-sites

Fig. 4

A Experimental design of the exposure of non-resistant E. coli to both streptomycin and kasugamycin. (Created in BioRender. Novoa, E. (2024) BioRender.com/b62y778). B NanoConsensus scores along the 16S rRNA (upper panel) and 23S rRNA (lower panel) of E. coli samples collected after 1 h or 16 h exposure to streptomycin (left panel) or kasugamycin (right panel). In gray, non-significant positions; in blue, regions identified by NanoConsensus in only one replicate; in red, regions identified in both replicates. Known modified sites found in replicable differentially modified regions identified by Nanoconsensus are also shown. Source data are provided as a Source Data file. C 3D structure of the bacterial ribosome (PDB: 7K00), highlighting the differentially modified rRNA residues upon streptomycin (upper panel) or kasugamycin (lower panel) exposure identified in this work. Differential rRNA modified residues are shown in red; in black, the antibiotic; in light green and orange, the tRNA molecule located in the A and P-sites, respectively.

Back to article page