Fig. 3: Tank/Azi2^DKO mice have dysregulated antibody production.

a Titers of different antibody isotypes in the blood plasma from 20-24-week-old mice of the indicated genotype (n = 5 for WT and Azi2^−/−, 6 for Tank^−/− and Tank/Azi2^DKO), mean + SEM, one-way ANOVA (p-value is indicated) with Tukey’s post-tests. b Representative photograph and weight of spleens isolated from 8-10-week-old WT and Tank/Azi2^DKO mice (n = 4 per group), mean + SEM, unpaired two-tailed t-test. c H&E staining of spleen tissue sections from 20-24-week-old mice of the indicated genotype. Representative images are based on analyzing samples from five mice per group. d, e Flow cytometry measurement of cellularity and viability of splenocytes from 8-12-week-old mice of the indicated genotype (d), followed by the analysis of splenic myeloid cells (CD3⁻, CD19⁻, NK1.1⁻, CD11b⁺) separated into macrophages (CD11c⁻, Ly6G⁻), neutrophils (CD11c⁻, Ly6G⁺), and dendritic cells (DC) (CD11c⁺) (e) (n = 8 per group), mean + SEM, one-way ANOVA (p-value is indicated) with Tukey’s post-tests. f–i Flow cytometry analysis of B cells from the spleen (f–g) and lymph nodes (h, i) from 8-12-week-old mice (n = 8 per group). The proportion of B cells (CD19⁺) (f, h) and the percentage of isotype-switched B cells (IgM⁻, IgD⁻) in organs isolated from the indicated mouse strains, mean + SEM, one-way ANOVA (p-value is indicated) with Tukey’s post-tests (g, i). n.s., not significant. The scale bar is 100 µm.