Fig. 6: NbREM1.1 negatively regulates ToCV infection.
a Accumulation of ToCV CP was analysed by western blot with an anti-CP antibody. Ponceau staining (Ponceau S) revealed equal protein loading in each lane. N. benthamiana plants were infiltrated with Agrobacterium containing empty vector (EV) as the mock control. b The ToCV RNA expression level was quantified by qRT-PCR using ToCV CP gene-specific primers. Data are presented as means ± SDs and were analysed using Student’s t-test (one-sided, P = 0.0224, 0.0033, respectively). Each treatment had three biological replicates. *P < 0.05, **P < 0.01. c Callose staining with aniline blue in WT, OE-REM1 and ΔNbrem1 plants (scale bar  = 20 μm). PDLP5-RFP served as a PD marker. d The relative intensity of callose staining in (c). The relative callose staining intensity was set as 1 in wild-type. Value is the mean ± SD (one-way ANOVA with Tukey’s test, n = 3 biologically independent experiments, P-values are shown in the Source Data file). Different lowercase letters indicate significant differences (P < 0.05). e. Fluorescence images of PVX-GFP in WT, OE-REM1 and ΔNbrem1 plants coexpressing EV, P7-Flag, P7S59D-Flag or P7S59A-Flag. GFP fluorescent signals were recorded by confocal microscopy at 3 dpi (scale bar = 100 μm). f Statistical analysis of PVX-GFP movement efficiency shown in (e). Value is the mean ± SD (one-way ANOVA with Tukey’s test, n = 5 biologically independent experiments, P-values are shown in the Source Data file). Different lowercase letters indicate significant differences (P < 0.05). For box plot, the horizontal lines from top to bottom represent the maximum, first quartile, median, third quartile, and minimum of the total data, respectively. Source data are provided as a Source Data file.
