Fig. 1: Divergent sub-families of DUF4123 proteins have co-evolved with distinct T6SS effector classes.

A Genomic context of representative DUF4123 proteins belonging to each identified gene neighborhood cluster. Neighboring genes are colored according to the effector family, indicated by the legend. DUF4123 proteins are colored in blue, and VgrG proteins are colored in gray. B Phylogenetic distribution of 1869 DUF4123 proteins encoded by the genera Burkholderia, Enterobacter, Escherichia, Pseudomonas, Salmonella, Serratia, Shigella, Vibrio, and Yersinia. Colors correspond to gene neighborhood clusters as indicated by the legend.