Fig. 4: A structurally conserved N-terminal lobe in DUF4123 proteins mediates VgrG binding. | Nature Communications

Fig. 4: A structurally conserved N-terminal lobe in DUF4123 proteins mediates VgrG binding.

From: A widespread accessory protein family diversifies the effector repertoire of the type VI secretion system spike

Fig. 4

A AlphaFold3 models of the indicated proteins. Gray shapes represent the N- and C-terminal domains as indicated. B, C AlphaFold3 model of the complexes formed between the indicated proteins. The C-terminal HTH motifs of VgrG6 and VgrG14 are colored yellow and green, respectively. D, E Molecular contacts formed at the predicted interface between the indicated proteins and western blot analysis of co-purification experiments between the indicated heterologously expressed tagged (hexahistidine (H) or FLAG (F)) proteins. Western blots are representative of three independent experiments.

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