Fig. 5: Behavioral tests in 1-month-old WT and Gpatch11Δ5/Δ5 mice and immunohistochemistry analysis of GPATCH11 on murine floating brain sections.
From: GPATCH11 variants cause mis-splicing and early-onset retinal dystrophy with neurological impairment
a Episodic memory assessment based on the Novel Object Recognition (NOR). Preference and Discrimination Indexes measurements. Behavioral data were estimated to be statistically significant when p ≤ 0.05 by One-tailed Student’s t-test. b Associative memory assessment using the Contextual Fear Conditioning (CFC). Percentage of freezing time index measured during training and testing phases, respectively. Behavioral data were estimated to be statistically significant when p ≤ 0.05 by Two-tailed Student’s t-test. Bars represent means ± SEM from 20 mice per genotype. ****p ≤ 0.0001. Boxplots show 25–75 percentiles and median; whiskers represent min and max values. c Immunostaining of floating brain sections of wildtype (WT) and Gpatch11Δ5/Δ5 with anti-GPATCH11 (magenta) and anti-CTIP2 (cyan) antibodies with a zoom on hippocampus. DAPI is used to label the cell nucleus (blue). Scale bars, 1 mm and 200 µm, respectively. Source data are provided as a Source Data file.
