Fig. 1: Nuclear wrinkling is modulated by stiffness of the extracellular matrix (ECM). | Nature Communications

Fig. 1: Nuclear wrinkling is modulated by stiffness of the extracellular matrix (ECM).

From: Matrix stiffness drives drop like nuclear deformation and lamin A/C tension-dependent YAP nuclear localization

Fig. 1

a Confocal images of stained DNA (blue) and lamin B1 (LMNB1, green) in human cancer cells cultured on soft, intermediate (int.) stiff, or stiff hydrogels. The scale bar is 5 μm. b Fluorescence lifetime imaging microscopy (FLIM) images of MDCK cells expressing lamin A/C strain sensor (Lamin-SS) and truncated control sensor (Lamin-TM)50; cytochalasin D treatment (cyto D) induces wrinkles. The scale bar is 10 μm. c Plot compares donor fluorescence lifetimes between control (smooth) and cytochalasin D-treated nuclei (wrinkled). n = 12, 17, 13, 19 for the four groups shown on the x-axis. Error bars, SEM. p-values from the two-sided Mann–Whitney U-test. Quantification of d nuclear EFC ratio, e volume, f surface area, and g height is shown for BHY, HN, and MDA-MB-231 cells cultured on soft, intermediate stiff, or stiff hydrogels corresponding to (a). n = 50, 45, 44, 43, 50, 61 for the six groups shown on the x-axis, based on three replicates. Error bars, SEM. p-values from two-sided Mann–Whitney U-test. h The mean EFC ratio correlated with mean nuclear height is shown for BHY (red), HN (green), and MDA-MB-231 (blue) cells cultured on soft, intermediate stiff, or stiff hydrogels. n = 50, 45, 44, 43, 50, 61 for BHY soft, BHY int. stiff, HN soft, HN int. stiff, MDA-MB-231 soft, MDA-MB-231 stiff. Error bars, SEM.

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