Fig. 2: Dynamic changes in laminar wrinkling are driven by cell rounding or spreading.

a Confocal images of photobleached GFP-LMNA (gray) expressing fibrosarcoma cells and fibroblasts before and after adding trypsin. Arcs separated by a T-shape photobleaching pattern are labeled with white numbers. The scale bar is 5 μm. b Quantification of arc length is shown for GFP-LMNA expressing fibrosarcoma cells (top) and fibroblasts (bottom) at 0 s and 40 s after adding trypsin. n = 20 and 20 for fibrosarcoma cells and fibroblasts, respectively. The arc examples in (a) are labeled in red. c Confocal images of GFP-LMNA (green) expressing fibrosarcoma cells and fibroblasts spread on glass substrates or suspended with trypsin. The scale bar is 10 μm. Corresponding quantification of d nuclear volume, e surface area, and f EFC ratio is shown for spread and suspended GFP-LMNA expressing fibrosarcoma cells and fibroblasts. n = 33, 33, 31, 31 for the four groups shown on the x-axis, based on three replicates. Error bars, SEM. p-values from the two-sided Mann–Whitney U-test. g Time-lapse confocal images of a GFP-LMNA (green) expressing fibrosarcoma cell and fibroblast spreading after seeding. F-actin (magenta) was stained with SPY650-FastAct. The scale bar is 10 μm. h Corresponding quantification of mean nuclear EFC ratio (green) and cell spreading area (magenta), both normalized to the corresponding value in the first time-frame, is shown for spreading fibrosarcoma cells (top) and fibroblasts (bottom). n = 12 and 9 for fibrosarcoma cells and fibroblasts, respectively, based on three replicates. Error bars, SEM.