Fig. 5: Laminar wrinkling correlates inversely with YAP nuclear localization. | Nature Communications

Fig. 5: Laminar wrinkling correlates inversely with YAP nuclear localization.

From: Matrix stiffness drives drop like nuclear deformation and lamin A/C tension-dependent YAP nuclear localization

Fig. 5

a Confocal images of stained lamin B1 (LMNB1, green) and YAP (red) in BHY, HN, and MDA-MB-231 cells cultured on soft and stiff hydrogels. The scale bar is 10 μm. b Quantification of nuclear to cytoplasmic YAP intensity ratio, correlated to nuclear EFC ratio (c) normalized to the corresponding value on soft hydrogels, is shown for BHY (red), HN (green), and MDA-MB-231 (blue) cells cultured on soft and stiff hydrogels. n = 50, 45, 44, 43, 50, 61 for the six groups shown on the x-axis, based on three replicates. Error bars, SEM. p-values from the two-sided Mann–Whitney U-test. d Confocal images of stained YAP (red) and F-actin (magenta) in GFP-LMNA (green) expressing HT-1080 and HN cells cultured on 30 and 50 μm diameter fibronectin micropattern. The scale bar is 10 μm. e Quantification of nuclear to cytoplasmic YAP intensity ratio, correlated to nuclear EFC ratio (f) normalized to the corresponding value on 30 μm pattern, is shown for HT-1080 (red) and HN (green) cells cultured on 30 and 50 μm diameter fibronectin micropattern. n = 42, 40, 40, 43 for the four groups shown on the x-axis, based on three replicates. Error bars, SEM. p-values from the two-sided Mann–Whitney U-test.

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