Fig. 6: Lamin A/C is required for laminar surface tension and the sensitivity of YAP to laminar unfolding and cell spreading. | Nature Communications

Fig. 6: Lamin A/C is required for laminar surface tension and the sensitivity of YAP to laminar unfolding and cell spreading.

From: Matrix stiffness drives drop like nuclear deformation and lamin A/C tension-dependent YAP nuclear localization

Fig. 6

a Efficacy of siRNA-mediated knockdown of lamin A/C expression is shown for HT-1080 and HN cells. Expression of lamin A/C in siRNA-treated cells relative to control cells treated with scrambled siRNA (siSCRM) after normalization of all Ct values to GAPDH expression using the 2-∆∆Ct method. Data represents the mean from three biological replicates. Error bars, SD. b Immunoblotting of lamin A/C is shown for HT-1080 and HN cells transfected with siSCRM and siLMNA. c Confocal images of stained lamin B1 (green) in HT-1080 and HN cells transfected with siSCRM or siLMNA deforming around 5-μm-tall rhodamine-fibronectin-coated PDMS microposts (red). Nuclear outlines relative to the position of microposts are shown at the bottom. The scale bar is 5 μm. d Nuclear to cytoplasmic YAP intensity ratio, correlated to cell spreading area or nuclear EFC ratio, is shown for HT-1080 and HN cells cultured on soft and stiff hydrogels. n = 77, 83, 77, 73 for HT-1080 siSCRM, HT-1080 siLMNA, HN siSCRM, HN siLMNA, respectively. Slopes and R-squared values of linear fitting and p-values for slope comparisons using a one-tailed F-test are shown on the graphs.

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