Fig. 3: Functional analysis of single voxel proteomics data from the largest voxels (voxel size: 200 µm × 200 µm × 10 µm). | Nature Communications

Fig. 3: Functional analysis of single voxel proteomics data from the largest voxels (voxel size: 200 µm × 200 µm × 10 µm).

From: Robust collection and processing for label-free single voxel proteomics

Fig. 3

a Differentially expressed CD protein markers between the red and white pulp regions (two-sided t-test, FDR < 0.05, s0 = 0.1, n = 4 biological replicates). b Enriched signaling pathways between the red and white pulp regions (FDR < 0.05). c Antibody-based CODEX image of human spleen tissue slide to validate label-free DIA MS quantitation: CD8 (yellow), CD20 (red), CD4 (green), and CD163 (cyan). Prior to image data generation, CODEX imaging was extensively optimized with multiple combinations of antibodies on human spleen tissue slides to ensure high reproducibility. Source data are provided as a Source Data file.

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