Fig. 4: Proteome mapping of human spleen tissue at 100 µm spatial resolution.
From: Robust collection and processing for label-free single voxel proteomics
a Tissue sections before, during, and after LCM collection. b The number of quantified protein groups for each voxel across the mapping region. c The normalized protein expression across the mapping region for region-specific protein markers. d Principal component analysis (PCA) of all the tissue voxels based on protein expression. Data point colors: Blue, red and green correspond to white pulp (WP), red pulp (RP) and the mixed regions from the histology view, respectively. e Signaling pathway enrichment based on differentially expressed proteins between WP and RP regions (FRD < 0.05). f 2D maps of the enriched immune signaling pathways using the average abundance of proteins involved in these pathways. In total 42 voxels at the size of 100 µm × 100 µm × 10 µm were collected to cover one WP and adjacent RP regions, and then processed by wcSOP followed by DIA-MS analysis. Source data are provided as a Source Data file.
