Fig. 7: Regulation of the mitonuclear balance by the equilibrium of mitochondrial dynamics is essential for regeneration.

A Summary of the DEGs in multi-transcriptomic analysis. Genes are summarized with subcellular localization and annotated functions. Downregulated and upregulated genes are in blue and red, respectively. B Left: representative WB images of egfp;egfp RNAi, opa1;egfp RNAi, drp1;egfp RNAi, and opa1;drp1 RNAi planarians treated with puromycin (puro) or puromycin and cycloheximide (CHX). Ponceau staining was used to confirm equal protein loading. n  =  3 biological replicates for each condition; each biological replicate was the mixture of proteins of six RNAi worms. Right: Quantitative representation of puromycin incorporation observed in left WB images. The data are shown as the mean ± SEM. P-value was calculated by unpaired two-tailed Student’s t-test versus egfp;egfp RNAi controls. C Quantification of the relative expression level of UPR signals in opa1 RNAi animals compared with egfp RNAi animals. n = 3 biological replicates for each RNAi condition. The quantification is shown as mean ± SEM. P-value was calculated by unpaired two-tailed Student’s t-test versus egfp RNAi controls. D Representative live images show the block of regeneration after mrpl2 RNAi and uqcr10 RNAi. Scale bar, 500 μm. E Quantification of H3P⁺ cell density at indicated regeneration time points of egfp RNAi, mrpl2 RNAi, and uqcr10 RNAi animals. Besides uqcr10 RNAi animal samples at 48 hpa and 72 hpa (n = 4 biological replicates for each condition), n = 3 samples from each of the other RNAi conditions at each time point. The data are shown as the mean ± SEM. Unpaired two-tailed Student’s t-test was used to determine the significance of differences at each time point versus egfp RNAi controls. F Representative FISH images of anterior and posterior patterning markers at 72 hpa. n = 3 worms of biological replicates for each RNAi treatment. Scale bar, 50 μm.