Fig. 2: Cryo-EM structure of Cas9-sgRNA-nucleosome complex targeting linker DNA.

a The domain organization of S. pyogenes Cas9. Residues 178 to 297 and 766 to 924 were not included in the final model. b Cryo-EM density maps of the Cas9-sgRNA-nucleosome complex in DNA attached state. c Overall structure of the Cas9-sgRNA-nucleosome complex in DNA attached state.