Fig. 6: Cys180 mutant in GSDME impairs methylcobalamin intervention in vivo.
From: Methylcobalamin protects against liver failure via engaging gasdermin E
GSDME Cys mutant plasmids constructed in adenovirus vector were injected into Gsdme-/- mice via tail vein, a GSDME expression in mice liver was detected with immunoblotting analysis. Mice were orally given 10 mg/kg MeCbl daily after BDL surgery. b–e Serum ALT, AST, IL-1α and IL-1β levels in serum 5 days after BDL/sham, n = 6. f Co-IP analysis of overexpressed GSDME with cleaved-caspase-3 in Gsdme-/- liver. g, h Representative H&E sections of the liver (g) (scale bar = 50 μm). Damaged area was traced with black dash line and analyzed using Image J software (h, n = 6 representative section of six independent mice samples). Data are mean ± SEM of at least two independent experiments. Each point is an individual mouse, number is indicated, n = 6–10 mice/group. Blots are representative data of six independent samples (a, f). Analysis was done using two-way ANOVA (b–e, h). p < 0.05 is considered significant to indicated group and p values are provided in graphs, n.s=nonsignificant. Source data are provided as Source Data file.
