Fig. 1: Specific side-on orientation of SecYAEG-ND and SecYA(ΔPPXD)EG-ND observed by HS-AFM.
From: AFM observation of protein translocation mediated by one unit of SecYEG-SecA complex
a Typical HS-AFM images of SecYAEG-ND (upper panels) and SecYA(ΔPPXD)EG-ND (lower panels) on a mica surface. The AFM image height (nm) is shown by the color bar. Representative data from more than 10 independent experiments with similar results are shown. b Cross-sectional height profiles for SecA regions along the red lines shown in (a). c Schematic representation of the structural models of SecYAEG-ND (upper) and SecYA(ΔPPXD)EG-ND (lower) at 80° in (f), accompanied by conical AFM probes utilized in the generation of simulated AFM images. PPXD is highlighted in yellow, the rest of SecA in blue, and SecYEG in magenta. d Simulated AFM images of SecYAEG-ND (upper) in wide-open form and SecYA(ΔPPXD)EG-ND (lower) in (c). The simulated AFM image height (nm) is shown by the color bar. e Cross-sectional height profiles for SecA regions along the red lines shown in (d). f Averaged image correlation scores as a function of rotation angles between the actual HS-AFM and the simulated AFM images. Black arrowhead indicates the angle with the highest correlation score. Source data of the graphs are provided as a Source Data file.
