Fig. 1: AR-MPXV5 effectively elicits a humoral immune response in cynomolgus macaques.
From: A penta-component mpox mRNA vaccine induces protective immunity in nonhuman primates
a Schematic diagram of the multicomponent MPXV mRNA vaccine design (created with BioRender.com). Created in BioRender. Tong, L. (2024) https://BioRender.com/k15r044. b Immunization and challenge study schedule for cynomolgus macaques. Created in BioRender. Tong, L. (2024) https://BioRender.com/k15r044. n = 3 independent biological replicates (Sham), n = 4 independent biological replicates (AR-MPXV5). c MPXV antigen-specific IgG antibody responses determined by ELISA. Data are shown as the mean ± SEM. Statistical differences were analyzed by using two-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. The exact P values are: P = 0.0085 (M1R, AR-MPXV5 day 14 vs AR-MPXV5 day 42), P = 0.0091 (M1R, AR-MPXV5 day 28 vs AR-MPXV5 day 42), P = 0.0053 (M1R, Sham day 42 vs AR-MPXV5 day 42); P < 0.0001 (E8L, AR-MPXV5 day 14 vs AR-MPXV5 day 42, AR-MPXV5 day 28 vs AR-MPXV5 day 42, Sham day 42 vs AR-MPXV5 day 42); P = 0.0245 (A29L, AR-MPXV5 day 14 vs AR-MPXV5 day 42), P = 0.0195 (A29L, AR-MPXV5 day 28 vs AR-MPXV5 day 42), P = 0.0097 (A29L, Sham day 42 vs AR-MPXV5 day 42); P = 0.0002 (A35R, AR-MPXV5 day 14 vs AR-MPXV5 day 42), P = 0.0003 (A35R, AR-MPXV5 day 28 vs AR-MPXV5 day 42), P = 0.0001 (A35R, Sham day 42 vs AR-MPXV5 day 42); P = 0.0059 (B6R, AR-MPXV5 day 14 vs AR-MPXV5 day 42), P = 0.0061 (B6R, AR-MPXV5 day 28 vs AR-MPXV5 day 42), P = 0.0038 (B6R, Sham day 42 vs AR-MPXV5 day 42). d, e Neutralizing antibody responses against VACV (d) and MPXV (e) 42 days after initial immunization. Data are shown as the mean ± SEM. Statistical differences were analyzed by using two-tailed unpaired t test. *P < 0.05. P = 0.0339 (d), P = 0.0168 (e). Source data are provided as a Source Data file.
