Fig. 1: Molecular identities of CG/SMG-projecting SPNs.
From: Parallel labeled-line organization of sympathetic outflow for selective organ regulation in mice
a Schematic of two extreme projection patterns for two distinct TC types. b Schematic of retrograde tracing from the CG/SMG. CA central autonomic area, IC intercalated nucleus, IML intermediolateral cell column, D dorsal, L lateral. c Representative horizontal view of the labeled CG/SMG, based on N = 3 mice. tdT tdTomato, DBH dopamine-β-hydroxylase, SMA superior mesenteric artery. Scale bar, 200 μm. d Light-sheet microscopic view of tissue-cleared SC displaying tdT+ cells (magenta). Scale bar, 500 μm. T thoracic. Number (e) and percentage (f) of tdT+ cells in the CA, IC, and IML for each SC segment. N = 3 mice. L lumbar. g Coronal sections of the T12 SC depicting tdT+ cells (magenta) and Chat (top) or nNos (bottom) immunostaining (green), with magnified images within white boxes. The arrows indicate tdT+ Chat+ cells. Scale bars, 100 μm. h Percentage of Chat+ or nNos+ cells among tdT+ cells in the CA, IC, and IML. N = 3 mice. i UMAP representation of 19 Chat+ SPN clusters (left) and a heat map showing log-normalized expression of the nNos gene (right). j Identification of enriched differentially expressed genes and expression of nNos among nNoslow clusters. The dot size reflects the population size, while the heat map shows the log-normalized expression level. k Coronal sections of the T12 SC displaying tdT+ cells (magenta) retrogradely labeled from the CG/SMG and Cartpt (top) or Chodl (bottom) mRNA expression (green), with magnified images within the white box. Arrows indicate dual-labeled cells. Scale bars, 100 μm for low-magnification images and 50 μm for magnified images. l Percentage of Cartpt+ cells (left) or Chodl+ cells (right) among tdT+ cells. N = 3 mice, except for Chodl+ data in the CA region, where N = 2 mice. m Schematic summary of the data. Data are shown as mean ± standard deviation (SD). Source data are provided as a Source Data file. For more data, see Supplementary Fig. 1 and Supplementary Movies 1 and 2.
