Fig. 3: Chemogenetic activation of Cartpt+ and Oxtr+ SPNs induces c-Fos in distinct targets.
From: Parallel labeled-line organization of sympathetic outflow for selective organ regulation in mice
Schematics illustrating the experimental design (a) and time line (b). c Coronal section of the SC showing mCherry (mC) expression (magenta) along with Chat, Cartpt, or Oxtr expression (green). AAV8 hSyn-DIO-Gq-mCherry was used for labeling. The right graph displays quantification for efficiency (mC+/markers+) and specificity (marker+/mC+). d Coronal section of the SC showing c-Fos (green) and mC (magenta) at 2 h after CNO administration. The right graph shows the percentage of c-Fos+ cells among mC+ cells in CNO-treated Gq and mC control mice. e c-Fos expression (green) in the whole CG/SMG (top) and AM sections (bottom). Magenta denotes mC+ axons. The right graph shows the number of c-Fos+ cells (CG/SMG) and the percentage of c-Fos+ per DAPI+ cells (AM) in CNO-treated Gq and mC control mice. ***p < 0.001 by a two-sided Welch’s t-test (d, e). Insets in panels (c–e) show magnified and channel-separated images in the boxed area. Scale bars, 100 μm for low-magnification images (c, d), 200 μm for low-magnification images (e), and 50 μm for magnified images. The number of animals (N) is indicated in blue within the panel. Data are shown as mean ± SD. Source data are provided as a Source Data file. For more data, see Supplementary Figs. 3 and 4.
