Fig. 4: Activation of Cartpt+ SPNs reduces gastrointestinal motility.
From: Parallel labeled-line organization of sympathetic outflow for selective organ regulation in mice
Schematics illustrating the experimental design (a) and time line (b). GITT, gastrointestinal transit time. The right image displays a fecal pellet stained red through carmine red administration. c GITT in Chat-Cre, Cartpt-Cre, or Oxtr-Cre mice injected with AAV8-hSyn-DIO-Gq-mCherry or AAV8-hSyn-DIO-mCherry (for control), upon administration of saline (white bar) or CNO (colored bar). The results from the same mice are connected with a line. Statistical comparisons were made using two-way ANOVA with repeated measures; (Chat-Cre) solution effect, p < 0.01, AAV effect, p < 0.01, interaction effect, ns. ***p < 0.001 by a post hoc two-sided Welch’s t-test. (Cartpt-Cre) solution effect, p < 0.01, AAV effect, p < 0.01, interaction effect, p < 0.01. ***p < 0.001 by a post hoc two-sided Welch’s t-test. (Oxtr-Cre) interaction effect, ns. d Schematics showing the Gq-mediated activation of Cartpt+ SPNs under the celiac ganglionectomy (CGX) condition. e GITT in CNO-treated Cartpt-Cre mice injected with AAV8-hSyn-DIO-Gq-mCherry under intact (left) and CGX (right) conditions. Data of intact conditions are the same as panel (c). f GITT measured without food in Cartpt-Cre mice injected with AAV8-hSyn-DIO-Gq-mCherry. g Schematic showing selective activation of SPNs projecting to the CG/SMG by injecting AAV-hSyn-DIO-Gq-mCherry into the T8–12 SC, to which Cre is retrogradely introduced from the CG/SMG via AAVrg-hSyn-Cre in wild-type mice. h GITT measured based on the panel (e) scheme upon administration of saline (white) or CNO (purple). **p < 0.01, ***p < 0.001 by two-sided Welch’s t-test (e, f, h). The number of animals (N) is indicated in blue within the panel. Data are shown as mean ± SD. Source data are provided as a Source Data file. For more data, see Supplementary Fig. 5.
