Fig. 5: Activation of Oxtr+ SPNs increases blood glucose levels in male mice.
From: Parallel labeled-line organization of sympathetic outflow for selective organ regulation in mice
a Change in blood glucose levels (ΔBGL) in Chat-Cre, Cartpt-Cre, or Oxtr-Cre mice injected with AAV8-hSyn-DIO-Gq-mCherry into the T8–T12 spinal segments, upon administration of saline (gray) or CNO (black). Individual data points are depicted with dots, and the average is denoted by a line. Top graphs are for males; bottom graphs are for females. +1 denotes that one data point is an outlier. Statistical comparisons were made using two-way ANOVA. Only the Chat: Gq, male and Oxtr: Gq, male groups exhibited significance; solution effect, p < 0.05; time course effect, p < 0.01; interaction effect, p < 0.05. #, p < 0.05, ##, p < 0.01 by a post hoc two-sided Welch’s t-test with Bonferroni correction showing a significant difference between the saline and CNO groups. b Area under the curve (AUC) measurements for 0 to 140 min. Two-way ANOVA with repeated measures; (Chat-Cre) sex effect, p < 0.05, solution effect, p < 0.01, interaction effect, p < 0.01. *p < 0.05 by a post hoc two-sided Welch’s t-test. (Cartpt-Cre) interaction effect, ns. (Oxtr-Cre) sex effect, p < 0.05, solution effect, ns, interaction effect, p < 0.01. ***p < 0.001 by a post hoc two-sided Welch’s t-test. c Schematics illustrating the Gq-mediated activation of Oxtr + SPNs in male mice under adrenalectomy. d ΔBGL under adrenalectomy, upon administration of saline (gray) or CNO (black). e AUC measurements for 0 to 140 min. ns, non-significant by a two-sided Welch’s t-test. The number of animals (N) is indicated in blue within the panel. Data are shown as mean ± SD. Source data are provided as a Source Data file. For more data, see Supplementary Figs. 6 and 7.
