Fig. 7: Differential activation of Oxtr+ and Cartpt+ SPNs in response to glucoprivation.
From: Parallel labeled-line organization of sympathetic outflow for selective organ regulation in mice
a Schematic representation of the experimental design to induce glucoprivation. b Representative SC sections showing c-Fos (green) and Chat (gray) after saline (left) or 2-DG (right) injection. Arrows indicate dual-labeled cells. c The number of c-Fos+ cells within Chat+ cells in the T8–10 and T11–13 segments under saline or 2-DG-treated conditions. N = 6 mice. ***p < 0.001 by a two-sided Welch’s t-test. d Representative SC sections showing c-Fos (green), Oxtr (magenta), and Chat (blue) (left), or c-Fos (green) and Cartpt (magenta) (right) after 2-DG injection. Arrows indicate triple-labeled cells. e Fraction of c-Fos+ cells within Oxtr+ cells among Chat+ cells in the 2-DG-treated condition. N = 6 mice. f Fraction of Oxtr+ or Cartpt+ cells within c-Fos+ cells in the 2-DG-treated condition. N = 6 mice. **p < 0.01 by a two-sided Welch’s t-test. Insets in panels (b) and (d) show magnified and channel-separated images in the boxed area. Scale bars, 100 μm for low-magnification images and 50 μm for magnified images (b, d). Data are shown as mean ± SD. Source data are provided as a Source Data file.
